UCSF

Alcohol Feeding Protocol

Bing Gui 20120604

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Supplies

Items

Catalog #

Ethanol Rodent Liquid Diet (Shake and Pour) 4 Liters/Bag

F1258SP

Control Rodent Liquid Diet (Shake and Pour) 4 Liters/Bag

F1259SP

Maltose Dextrin (Dried Glucose Syrup, Fine)

3653

Tube Holders

9015

Liquid Diet Feeding Tubes 50 ml

9019

Autoclavable Screw Cap

9085

Fisherbrand Colored Label Tapes

 
  1. Determine the total number of mice maintained according to the experimental design.
  2. Order Ethanol and Control food as well as Maltose Dextrin, tubes, tube holders and some spare caps from BioServ.
  1. Use the feeding chart below to make ready-to-use liquid diet. The ready-to-use liquid diet has a shelf life of 3 days if kept in a refrigerator.

1 Control diet

Feeding

EtOH Kcal/L (g/L)

Dry mix (g)

Water (ml)

   

Total (ml)

All the time

0

225.5

860.0

   

1085.5

2 EtOH diet

Pre-feeding

EtOH Kcal/L (g/L)

Dry mix (g)

Water (ml)

95% EtOH (ml/L)

Maltose dextrin (g)

Total (ml)

2 days

0

133.0

957.0

0

 

1090.0

2 days

220

133.0

916.0

41

34

1085.5

2 days

260

133.0

908.0

49

23

1113.0

2 days

300

133.0

901.0

56

14

1104.0

Feeding

EtOH Kcal/L (g/L)

Dry mix (g)

Water (ml)

95% EtOH (ml/L)

Maltose dextrin (g)

Total (ml)

Week 1

338

133.0

894.0

63

4

1094.0

Week 2,3

352

133.0

891.0

66

0

1090.0

Week 4,5

366

133.0

889.0

68

0

1090.0

Week 6,7

380

133.0

886.0

71

0

1090.0

Week 8

400

133.0

882.0

75

0

1090.0

                 
  1. Make daily record of feeding amount to monitor the consumption. Ideally mice in the Control group and mice in the Ethanol group should consume similar amount of liquid food per day.

Record Form - Alcohol Feeding

3/5

Cage #

C1

C2

C3

C4

C5

C6

Mon

Tube #

T11

T12

T21

T22

T31

T32

T41

T42

T51

T52

T61

T62

 

Prepared (ml)

25

40

40

40

40

40

40

40

40

40

40

40

 

Leftover (ml)

5

5

0

0

10

10

0

0

10

15

0

0

 

Consumed (ml)

20

35

40

40

30

30

40

40

30

25

40

40

  1. Use Fisher Colored Label Tapes to seal the feeding tubes before refill the tubes. Change the food tubes and wash the dirty tubes daily.
  2. Feed the mice to 8 or 12 weeks according to the experimental design. Sarcrifice the mice and collect the tissues at the end of the feeding course.

Liquid diet preparation and feeding procedure

  1. Control diet:
    1. Dry mix: 225.5g
    2. Water: 860 ml
  2. Alcohol diet:
    Pre-feeding ETOH KCAL/L (G/L) Dry mix (g) Water (ml) 95% EtOH (ml/L) Maltose dextrin (g)
    2 days 0 133 957 0  
    2 days 220 133 916 41 34
    2 days 260 133 908 49 23
    2 days 300 133 901 56 14
    Pre-feeding ETOH KCAL/L (G/L) Dry mix (g) Water (ml) 95% EtOH (ml/L) Maltose dextrin (g)
    Week 1 338 (48) 133 894 63 4
    Week 2, 3 352 (50) 133 891 66 0
    Week 4, 5 366 (52) 133 889 68 0
    Week 6, 7 380 (54) 133 886 71 0
    Week 8 400 (57) 133 882 75 0

Product #F1259SP; Liquid Rat Diet, LD '82; Shake & Pour; Control

Directions for preparing 4 liters

  1. Add 1/3 of the dry mix, Product F1259SP (902.2 gm/bag) into the Shake & Pour Bottle.
  2. Measure 3440 ml. of warm tap water. Pour 113 of the warm tap water to dry mix into the Shake & Pour Bottle.
  3. Shake approximately 30 seconds.
  4. Add additional 113 of the diet and additional 1/3 of warm tap water into the Shake & Pour Bottle.
  5. Shake approximately 30 seconds.
  6. Add the remaining 113 of the diet and 113 warm tap water.
  7. Shake approximately 30 seconds.
  8. Refrigerate any unused portion.

For best results

  1. As a matter of convenience, the mixing directions and package quantities may be proportionately reduced or increased. For added convenience, use a bottle with pre-measured water level markings.
  2. If lumps occur, add the dry mix to the water in four increments of 25% each and shake the diet for one minute between each addition of dry mix.
  3. Under refrigeration, the sealed packages of dry mix have a shelf life of up to six (6) months.
  4. Shelf life of liquid diet: The prepared liquid diet should be maintained under refrigeration and should be dispensed within three (3) days. The prepared diet should not be kept longer than 48 hours at room temperature to avoid any spoilage. Invert bottle several times prior to dispensing to guard against possible settling in some complex formulations.

Product #F1258SP; Liquid Rat Diet, LD'82; Shake & Pour--Ethanol

Directions for preparing 4 liters

  1. Add II3 of the dry mix, Product Fl258SP (533.6gm/bag) into the Shake & Pour Bottle.
  2. Measure 3560 ml of warm tap water. Add II3 of warm water to dry mix in Shake & Pour Bottle. Shake approximately 30 seconds.
  3. Add additional 1/3 of the diet and then 1/3 of warm tap water into the Shake & Pour Bottle.
  4. Shake approximately 30 seconds.
  5. Add the remaining 1/3 of the diet and the remaining water into the Shake & Pour Bottle and shake approximately 30 seconds.
  6. Measure 268 m!. of 95% Ethanol (not included) and add to the Shake & Pour Bottle.
  7. Shake approximately 30 seconds.
  8. Refrigerate any unused portion.

NOTE: These directions are for preparing diets that yield 350 Kcal per liter of ETOH.

For best results

  1. As a matter of convenience, the mixing directions and package quantities may be proportionately reduced or increased. For added convenience, use a bottle with pre-measured water level markings. Use a scoop to remove the product.
  2. If lumps occur, add the dry mix to the water in four increments of 25% each and shake the diet for one minute between each addition of dry mix.
  3. Under refrigeration, the sealed packages of dry mix have a shelf life of up to six (6) months.
  4. Shelf life and feeding method:
    1. The prepared liquid diet should be maintained under refrigeration and should be dispensed within three (3) days.
    2. It is recommended that ETOH animals be gradually introduced to the ethanol diet as follows:

      1st--3rd Day 1/3 ETOH & 2/3 Control
      4th--7th Day 2/3 ETOH & l/3 Control
      8th Day on Pure ETOH diet.