Below are protocols used in our lab.
Jump below to
- Cell biology
- Molecular biology
- Bioinformatics analysis
- Biochemistry
- Histology
- Animal
- Other protocols
Cell biology
- Adhesion assay
- Amino Acid Assay.pdf
- Cell enumeration by crystal violet staining
- Cell migration assay
- Cell Migration and Invasion Assays.pdf
How to read the cell line STR Profiling Test Results- IF staining cell line
- In vitro hepatocytes transfection
- isolation_of_primary_liver_tumor_cells_2021_update.pdf
- Lentiviral transfection
- Lentiviral production
- lentivirus_production_y2020_update.pdf
- Mycoplasma detection (Nature)
- PI labeling for FAS analysis
- siRNA Transfection using RNAiMAX.pdf
- soft_agar_assay_y2020_update.pdf
Molecular biology
- Cloning
- digest map of Plasmids .docx
- Common primer sequences
- common used primer in Chen lab.docx
- CRISPR cloning notes and sgRNA design.pdf
- CRISPR cloning protocol Zhang Lab.pdf
- Extract DNA from paraffin blocks
- identify_sgrna_2022.pdf
- PX330 cloning protocol.docx
- QuantitativeMicrosatelliteAnalysis.doc
- RT-PCR
- shRNA template for PT3 cloning.docx
- TaqMan RT
- Thermoscript RT-PCR
Bioinformatics Analysis
Biochemistry
Histology
- BrdU staining
- H&E staining
- IF on frozen sections (double IF, for 2 rat antibodies)
- IF on frozen sections (double IF)
- IF paraffin slides (double staining)
- IF paraffin slides
- IHC rabbit antibody protocol
- IHC mouse antibody protocol
- Oil red O staining
- PicroSirius red staining
Animal
- Alcohol feeding protocol
- alpelisib_formulation.pdf
- blood_analysis_for_liver_panel.pdf
- Cabozantinib_formulation.pdf
- DEN protocol
- DDC protocol.pdf
- Gemcitabine formulation
- Genotyping
- Harvest mouse liver tissues
- Hydrodynamic tail vein injection
- INK128 formulation
- liver_tumor_organoid_protocol.pdf
- Rapacymin mice
- spleen_injection.pdf
- Sorefanib formulation
- Tamoxifen injection
- Tunicamycin treatment