Protocols
Below are protocols used in our lab.
Jump below to
- Cell biology
- Molecular biology
- Bioinformatics analysis
- Biochemistry
- Histology
- Animal
- Other protocols
Cell biology
- Adhesion assay
Amino Acid Assay- Cell enumeration by crystal violet staining
- Cell migration assay
- Cell Migration and Invasion Assays
How to read the cell line STR Profiling Test Results- IF staining cell line
- In vitro hepatocytes transfection
- Isolation of primary liver tumor cells, 2021 update
- Lentiviral transfection
- Lentiviral production
- lentivirus_production_y2020_update.pdf
- Mycoplasma detection (Nature)
- PI labeling for FAS analysis
- siRNA Transfection using RNAiMAX
- soft_agar_assay_y2020_update.pdf
Molecular biology
- Cloning
digest map of Plasmids- Common primer sequences
- common used primer in Chen lab
- CRISPR cloning notes and sgRNA design
- CRISPR cloning protocol Zhang Lab
- Extract DNA from paraffin blocks
- Identify sgRNA 2022.pdf
- PX330 cloning protocol
- Quantitative Microsatellite Analysis
- RT-PCR
- shRNA template for PT3 cloning
- TaqMan RT
- Thermoscript RT-PCR
Bioinformatics Analysis
Biochemistry
Histology
- BrdU staining
- H&E staining
- IF on frozen sections (double IF, for 2 rat antibodies)
- IF on frozen sections (double IF)
- IF paraffin slides (double staining)
- IF paraffin slides
- IHC rabbit antibody protocol
- IHC mouse antibody protocol
- Oil red O staining
- PicroSirius red staining
Animal
- Alcohol feeding protocol
- alpelisib_formulation.pdf
- Blood analysis for liver panel
- cabozantinib_formulation.pdf
- DEN protocol
- DDC protocol
- Gemcitabine formulation
- Genotyping
- Harvest mouse liver tissues
- Hydrodynamic tail vein injection
- INK128 formulation
- liver_tumor_organoid_protocol.pdf
- Rapacymin mice
- spleen_injection.pdf
- Sorefanib formulation
- Tamoxifen injection
- Tunicamycin treatment