UCSF

DEN Protocol

DEN‐induced HCC model in male mice

Zhong Xu, January 2016

Ordering information

Items Catalog # MFR
DEN N0756-10 ML Sigma
HFD 14726603 Fisher Scientific Company, LLC

Protocol

  1. prepared 5 ug/ul DEN working solution (26.4 μl DEN +5 ml sterilized PBS)
    [26.4 ul DEN =25 .08 mg DEN (density=0.95, Mass= Volume X Density)]
  2. The mice were injected intraperitoneally with DEN (25 mg/kg) at 14‐day age.
  3. Wean and keep male mice at 21‐day age.
  4. Identify gene type (for instance ACR+, ACR‐) by genotyping.
  5. After 4 weeks, mice at 6 weeks age were fed with HFD.
  6. At 33 weeks, mice were scarified for H&E, IHC and Western blot, etc.

Example

Mice at 6 weeks age were separated into two groups. One group was fed with standard diet, another was fed with HFD. Then mice were scarified at 33 weeks.

HFD group

hfd1
hfd2

SD group

sd1
sd2