UCSF

Tamoxifen Injection

Tamoxifen (TM) in corn oil:

  1. Dissolve 20mg/ml Tamoxifen in corn oil for 1 hr in a roller (hybridization oven) at 65C (protect tube from light with tin foil)
  2. Keep your stock at +4C for up to a week
  3. Before injecting, warm Tamoxifen at 65C for 10min
  4. Use a 27-gauge needle and inject the mouse with 9mg/40gr body weight, 3x times, one every other day (you can do up to 5 injections continuously)
  5. Determine recombination one week after the last TM injection

For pregnant females:

  1. Inject once with 3mg/40gr body weight.
  2. C. Section embryos and transfer them to a foster mother

Tamoxifen minimum 99%: Sigma Cat #T5648

Corn oil: Sigma Cat# C8267

TM administration at 9mg/40gr b.w. (20mg/ml stock)

Weight (gr) Volume TM (ul)
7 79
8 90
9 101
10 112
11 124
12 135
13 146.5
14 157.5
15 169
16 180
17 191.5
18 202.5
19 214
20 225
21 236.5
22 247.5
23 258
24 270
25 281
26 292.5
27 303.5
28 315
29 326
30 337
31 349
32 360
33 371
34 382.5
35 394
36 405
37 416
38 427.5
39 439
40 450
41 461