Sorafenib Formulation

For in vivo experiments: For a concentration of 30 mg/Kg (BW:25g):

1. Heat Cremophor EL at 60°C

2. Dissolve 100 mg of Sorafenib in 2.5mL of a stock solution containing 75% Ethanol and Cremophor EL（1:1）at 60°C (40mg/mL).

3. Vortex at highest speed and put back on 60°C, until Sorafenib is completely dissolved (This process can take up to 15 min).Freeze aliquots and store at -80°C.

4. Dilute the stock solution 1:4 in water and applicate 100 uL orally gavage for each mouse.

5. Note: The sorafenib will flocculate from diluted solution after one or two hours. You need start all over again for each dilute.

For a concentration of 100 mg/Kg (BW:25g):

1. Heat Cremophor EL at 60°C

2. Solve 250 mg of Sorafenib in 10 mL of a stock solution containing 75% Ethanol and Cremophor EL（1:1）at 60°C (25mg/mL).

3. Vortex at highest speed and put back on 60°C, until Sorafenib is completely solved (This process can take up to 20 min). Store at -80°C.

4. Applicate 100 uL orally gavage for each mouse.

Products information

Sorafenib： Sorafenib, p-Toluenesulfonate Salt, >99%（Cat.NO.:S-8502, LC Laboratories）

EL: Cremophore EL(Lot #: BCBK9801V, Sigma Life Science)

Ethanol(90%): Ethanol, HPLC Grade, 90%( Lot #: 44134, Alfa Aesar)

Ethanol(75%): 25 mL Ethanol(90%) + 5 mL H20

For in vitro experiments

Dissolve Sorafenib in DMSO to the concentration of 20mM/L. For long term storage, freeze aliquots and store at -80.