IF Staining Cell Line
- Grow cell in wells.
- Wash with PBS TWICE (500ul/well) .
- Fix with 200ul of 4% paraformaldehyde/PBS for 20 min at RT (fix time)
(Stock is 16%).
- Permeabilize with 0.3% Triton x-100/PBS for 15 min at RT (fix time).
- Treated with goat serum/PBS (1:200) for 30 min with shaker ~21 at RT.
- Added anti-beta-catenin Ab in goat serum/PBS for 1.5 hr at RT (fix time)
- Wash with PBS three times for 5 min each with shaker ~21 at RT
- Added 100ul goat anti-mouse FITC(green, 1:500) for 45 min at RT. (fix time)
- Wash with PBS three times for 10 min each at RT.