ALPINE (α-Amine Ligation Profiling Informing N-terminal modification Enzyme selection​) is a web-based tool that enables algorithmic selection of subtiligase mutants for protein and peptide N-terminal modification applications, including site-specific bioconjugation and N-terminomics studies.


The status of the α-aminome, or all protein N-termini, at a given time in a cell, can provide a wealth of information regarding protein turnover, protein trafficking, and protease signaling. The Degrabase aims to provide a non-biased description of all possible caspases substrates found in healthy and apoptotic human cells and shares with the scientific community this valuable dataset, which contains over 8,000 unique α-amines detected with high confidence using mass spectrometry and our degradomics methodology.