UCSF

We genetically encode unnatural amino acids in live cells and organisms to investigate biological processes in their natural settings and to develop new biotherapeutics.

Selected publications

1. Cao, L.; Wang, L. New Covalent Bonding Ability for Proteins. Protein Sci. 31: 312-322 (2022). PubMed

2. Li, Q., Chen, Q., Klauser, P. C., Li, M., Zheng, F., Wang, N., Li, X., Zhang, Q., Fu, X., Wang, Q., Xu, Y., Wang, L. Developing Covalent Protein Drugs via Proximity-Enabled Reactive Therapeutics. Cell. 182(1): 85-97 (2020). PubMed

3. Wang, N., Yang, B., Fu, C., Zhu, H., Zheng, F., Kobayashi, J., Liu J., Li, S., Cheng, M., Wang, P. G., Wang, Q., Wang, L. Genetically encoding fluorosulfate-L-tyrosine to react with lysine, histidine and tyrosine via SuFEx in protein in vivo. J. Am. Chem. Soc. 140(15):4995-4999 (2018). PubMed

4. Yang, B., Tang, S., Ma, C., Li, S. T., Shao, G. C., Dang, B., DeGrado, W. F., Dong, M. Q., Wang, P. G., Ding, S., Wang, L. Spontaneous and specific chemical crosslinking in live cells to capture and identify protein interactions. Nat. Comm. 8:1-10 (2017). PubMed

5. Hoppmann, C., Wong, A., Yang, B., Li, S., Hunter, T., Shokat, K.M., and Wang, L. Site-specific incorporation of phosphotyrosine using an expanded genetic code. Nat. Chem. Biol. 13:842-844 (2017). PubMed

6. Chen, Y., Ma, J., Lu, W, Tian, M., Thauvin, M., Yuan, C., Volovitch, M., Wang, Q., Holst, J., Liu, M., Vriz, S., Ye, S., Wang, L., Li, D. Heritable expansion of the genetic code in mouse and zebrafish. Cell Research 27:294-297 (2017). PubMed

7. Coin I, Katritch V, Sun T, Xiang Z, Siu FY, Beyermann M, Stevens RC, Wang L. Genetically encoded chemical probes in cells reveal the binding path of urocortin-I to CRF class B GPCR. Cell. 2013 Dec 5; 155(6):1258-69. PubMed

8. Kang JY, Kawaguchi D, Coin I, Xiang Z, O'Leary DD, Slesinger PA, Wang L. In vivo expression of a light-activatable potassium channel using unnatural amino acids. Neuron. 2013 Oct 16; 80(2):358-70. PubMed

9. Xiang Z, Ren H, Hu YS, Coin I, Wei J, Cang H, Wang L. Adding an unnatural covalent bond to proteins through proximity-enhanced bioreactivity. Nat. Methods. 2013 Sep; 10(9):885-8. PubMed

10. Johnson DB, Xu J, Shen Z, Takimoto JK, Schultz MD, Schmitz RJ, Xiang Z, Ecker JR, Briggs SP, Wang L. RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites. Nat. Chem. Biol. 2011 Nov; 7(11):779-86. PubMed

11. Wang W, Takimoto JK, Louie GV, Baiga TJ, Noel JP, Lee KF, Slesinger PA, Wang L. Genetically encoding unnatural amino acids for cellular and neuronal studies. Nat. Neurosci. 2007 Aug; 10(8):1063-72. PubMed

12. Wang L. Expanding the genetic code. Science. 2003 Oct 24; 302(5645):584-5. PubMed

13. Wang L, Brock A, Herberich B, Schultz PG. Expanding the genetic code of Escherichia coli. Science. 2001 Apr 20; 292(5516):498-500. PubMed