Research laboratory of Lei Wang, PhD
Chemical Biology Laboratory

Publications

  1. Hoppmann, C., Wong, A., Yang, B., Li, S., Hunter, T., Shokat, K.M., and Wang, L.* Site-specific incorporation of phosphotyrosine using an expanded genetic code. Nat. Chem. Biol. (2017) in press. DOI: 10.1038/nchembio.2406

    View in: PubMed

  2. Wang, L.* Genetically encoding new bioreactivity. N. Biotechnol. 38:16-25 (2017)

    View in: PubMed

  3. Klippenstein V, Hoppmann C, Ye S, Wang L, Paoletti P. Optocontrol of glutamate receptor activity by single side-chain photoisomerization. Elife 6:e25808 (2017).

    View in: PubMed

  4. Chen, Y., Ma, J., Lu, W, Tian, M., Thauvin, M., Yuan, C., Volovitch, M., Wang, Q., Holst, J., Liu, M., Vriz, S., Ye, S.*, Wang, L.*, Li, D.* Heritable expansion of the genetic code in mouse and zebrafish. Cell Research 27:294-297 (2017).

    View in: PubMed

    Science

    Editor’s Summary (Science 2017, 355:36)

  5. Pentelute BL, Wang L. Editorial overview: Chemistry for biopolymers to investigate and even move beyond nature. Curr Opin Chem Biol. 2016 Oct 14. PMID: 27751659.

    View in: PubMed

  6. Kobayashi T, Hoppmann C, Yang B, Wang L.* Using Protein-Confined Proximity to Determine Chemical Reactivity. J Am Chem Soc. 2016 Oct 31; 138(45):14832-14835.

    View in: PubMed

  7. Ren H, Yang B, Ma C, Hu YS, Wang PG, Wang L.* Cysteine Sulfoxidation Increases the Photostability of Red Fluorescent Proteins. ACS Chem Biol. 2016 Oct 21; 11(10):2679-2684.

    View in: PubMed

  8. Alamudi SH, Satapathy R, Kim J, Su D, Ren H, Das R, Hu L, Alvarado-Martínez E, Lee JY, Hoppmann C, Peña-Cabrera E, Ha HH, Park HS, Wang L, Chang YT, Development of background-free tame fluorescent probes for intracellular live cell imaging. Nat Commun. 2016; 7:11964-. PMID: 27321135.

    View in: PubMed

    F1000Prime Recommended

  9. Kang, J.-Y., Kawaguchi, D., and Wang, L.* Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons. J. Vis. Exp. 109, e53818, doi:10.3791/53818 (2016).

    View in: PubMed

  10. Hoppmann C, Wang L.* Proximity-enabled bioreactivity to generate covalent peptide inhibitors of p53-Mdm4. Chem Commun (Camb). 2016 Apr 14; 52(29):5140-5143.

    View in: PubMed

  11. Hoppmann, C., Maslennikov I, Choe S, Wang L.* In Situ Formation of an Azo Bridge on Proteins Controllable by Visible Light. J Am Chem Soc. 2015 Sep 9; 137(35):11218-21.

    View in: PubMed

  12. Li YC, Rodewald LW, Hoppmann C, Wong ET, Lebreton S, Safar P, Patek M, Wang L, Wertman KF, Wahl GM. A Versatile Platform to Analyze Low-Affinity and Transient Protein-Protein Interactions in Living Cells in Real Time. Cell Rep. 2014 Dec 11; 9(5):1946-58.

    View in: PubMed

  13. Chen XH, Xiang Z, Hu YS, Lacey VK, Cang H, Wang L. Genetically encoding an electrophilic amino acid for protein stapling and covalent binding to native receptors. ACS Chem Biol. 2014 Sep 19; 9(9):1956-61.

    View in: PubMed

    F1000Prime Recommended

  14. Wang Q, Sun T, Xu J, Shen Z, Briggs SP, Zhou D, Wang L. Response and adaptation of Escherichia coli to suppression of the amber stop codon. Chembiochem. 2014 Aug 18; 15(12):1744-9.

    View in: PubMed

    Nature Chemical Biology

    Research Highlights (Nat. Chem. Biol. 2014, 10:695)

  15. Hoppmann C, Lacey VK, Louie GV, Wei J, Noel JP, Wang L. Genetically Encoding Photoswitchable Click Amino Acids in Escherichia coli and Mammalian Cells. Angew Chem Int Ed Engl. 2014 Apr 7; 53(15):3932-6.

    View in: PubMed

  16. Xiang Z, Lacey VK, Ren H, Xu J, Burban DJ, Jennings PA, Wang L. Proximity-enabled protein crosslinking through genetically encoding haloalkane unnatural amino acids. Angew Chem Int Ed Engl. 2014 Feb 17; 53(8):2190-3.

    View in: PubMed

  17. Coin I, Katritch V, Sun T, Xiang Z, Siu FY, Beyermann M, Stevens RC, Wang L. Genetically encoded chemical probes in cells reveal the binding path of urocortin-I to CRF class B GPCR. Cell. 2013 Dec 5; 155(6):1258-69.

    View in: PubMed

    Cell

    Leading Edge Previews (Cell, 2013, 155:1207)

    F1000Prime Recommended

  18. Kang JY, Kawaguchi D, Coin I, Xiang Z, O'Leary DD, Slesinger PA, Wang L. In vivo expression of a light-activatable potassium channel using unnatural amino acids. Neuron. 2013 Oct 16; 80(2):358-70.

    View in: PubMed

  19. Lacey VK, Louie GV, Noel JP, Wang L. Expanding the library and substrate diversity of the pyrrolysyl-tRNA synthetase to incorporate unnatural amino acids containing conjugated rings. Chembiochem. 2013 Nov 4; 14(16):2100-5.

    View in: PubMed

  20. Xiang Z, Ren H, Hu YS, Coin I, Wei J, Cang H, Wang L. Adding an unnatural covalent bond to proteins through proximity-enhanced bioreactivity. Nat Methods. 2013 Sep; 10(9):885-8.

    View in: PubMed

    F1000Prime Recommended

  21. Jagadish K, Borra R, Lacey V, Majumder S, Shekhtman A, Wang L, Camarero JA. Expression of fluorescent cyclotides using protein trans-splicing for easy monitoring of cyclotide-protein interactions. Angew Chem Int Ed Engl. 2013 Mar 11; 52(11):3126-31.

    View in: PubMed

  22. Johnson DB, Wang C, Xu J, Schultz MD, Schmitz RJ, Ecker JR, Wang L. Release factor one is nonessential in Escherichia coli. ACS Chem Biol. 2012 Aug 17; 7(8):1337-44.

    View in: PubMed

  23. Parrish AR, She X, Xiang Z, Coin I, Shen Z, Briggs SP, Dillin A, Wang L. Expanding the genetic code of Caenorhabditis elegans using bacterial aminoacyl-tRNA synthetase/tRNA pairs. ACS Chem Biol. 2012 Jul 20; 7(7):1292-302.

    View in: PubMed

    F1000Prime Recommended

  24. Wang Q, Wang L. Genetic incorporation of unnatural amino acids into proteins in yeast. Methods Mol Biol. 2012; 794:199-213.

    View in: PubMed

  25. Johnson DB, Xu J, Shen Z, Takimoto JK, Schultz MD, Schmitz RJ, Xiang Z, Ecker JR, Briggs SP, Wang L. RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites. Nat Chem Biol. 2011 Nov; 7(11):779-86.

    View in: PubMed

    F1000Prime Recommended

  26. Shen B, Xiang Z, Miller B, Louie G, Wang W, Noel JP, Gage FH, Wang L. Genetically encoding unnatural amino acids in neural stem cells and optically reporting voltage-sensitive domain changes in differentiated neurons. Stem Cells. 2011 Aug; 29(8):1231-40.

    View in: PubMed

  27. Lacey VK, Parrish AR, Han S, Shen Z, Briggs SP, Ma Y, Wang L. A fluorescent reporter of the phosphorylation status of the substrate protein STAT3. Angew Chem Int Ed Engl. 2011 Sep 5; 50(37):8692-6.

    View in: PubMed

    Angewandte Chemie

    Editor’s Hot Paper

  28. Coin I, Perrin MH, Vale WW, Wang L. Photo-cross-linkers incorporated into G-protein-coupled receptors in mammalian cells: a ligand comparison. Angew Chem Int Ed Engl. 2011 Aug 22; 50(35):8077-81.

    View in: PubMed

    Angewandte Chemie

    VIP (Very Important Paper)

  29. Xiang Z, Wang L. Enantiospecific synthesis of genetically encodable fluorescent unnatural amino acid L-3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid. J Org Chem. 2011 Aug 5; 76(15):6367-71.

    View in: PubMed

  30. Takimoto JK, Dellas N, Noel JP, Wang L. Stereochemical basis for engineered pyrrolysyl-tRNA synthetase and the efficient in vivo incorporation of structurally divergent non-native amino acids. ACS Chem Biol. 2011 Jul 15; 6(7):733-43.

    View in: PubMed

  31. Parrish AR, Wang L. Genetic Incorporation of Unnatural Amino Acids into Proteins. Comprehensive Natural Products Chemistry II Chemistry and Biology. 2010; 5:587-617.

  32. Takimoto JK, Xiang Z, Kang JY, Wang L. Esterification of an unnatural amino acid structurally deviating from canonical amino acids promotes its uptake and incorporation into proteins in mammalian cells. Chembiochem. 2010 Nov 2; 11(16):2268-72.

    View in: PubMed

  33. Johnson DB, Wang L. Imprints of the genetic code in the ribosome. Proc Natl Acad Sci U S A. 2010 May 4; 107(18):8298-303.

    View in: PubMed

    Science

    Editors’ Choice (Science, 2010, 328:407)

  34. Majmudar CY, Lee LW, Lancia JK, Nwokoye A, Wang Q, Wands AM, Wang L, Mapp AK. Impact of nonnatural amino acid mutagenesis on the in vivo function and binding modes of a transcriptional activator. J Am Chem Soc. 2009 Oct 14; 131(40):14240-2.

    View in: PubMed

  35. Takimoto JK, Adams KL, Xiang Z, Wang L. Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells. Mol Biosyst. 2009 Sep; 5(9):931-4.

    View in: PubMed

  36. Wang Q, Parrish AR, Wang L. Expanding the genetic code for biological studies. Chem Biol. 2009 Mar 27; 16(3):323-36.

    View in: PubMed

  37. Shu X, Wang L, Colip L, Kallio K, Remington SJ. Unique interactions between the chromophore and glutamate 16 lead to far-red emission in a red fluorescent protein. Protein Sci. 2009 Feb; 18(2):460-6.

    View in: PubMed

  38. Lin MZ, Wang L. Selective labeling of proteins with chemical probes in living cells. Physiology (Bethesda). 2008 Jun; 23:131-41.

    View in: PubMed

  39. Johnson DBF, Takimoto JK, Xu J, Wang L. Unnatural Amino Acids to Investigate Biological Processes. Wiley Encyclopedia of Chemical Biology. 2008; 10.1002/9780470048672.wecb585.

  40. Wang Q, Wang L. New methods enabling efficient incorporation of unnatural amino acids in yeast. J Am Chem Soc. 2008 May 14; 130(19):6066-7.

    View in: PubMed

  41. Wang W, Takimoto JK, Louie GV, Baiga TJ, Noel JP, Lee KF, Slesinger PA, Wang L. Genetically encoding unnatural amino acids for cellular and neuronal studies. Nat Neurosci. 2007 Aug; 10(8):1063-72.

    View in: PubMed

    F1000Prime Recommended

  42. Parrish AR, Wang W, Wang L. Manipulating proteins for neuroscience. Curr Opin Neurobiol. 2006 Oct; 16(5):585-92.

    View in: PubMed

  43. Wang L, Xie J, Schultz PG. Expanding the genetic code. Annu Rev Biophys Biomol Struct. 2006; 35:225-49.

    View in: PubMed

  44. Wang L, Tsien RY. Evolving proteins in mammalian cells using somatic hypermutation. Nat Protoc. 2006; 1(3):1346-50.

    View in: PubMed

  45. Zhang Y, Wang L, Schultz PG, Wilson IA. Crystal structures of apo wild-type M. jannaschii tyrosyl-tRNA synthetase (TyrRS) and an engineered TyrRS specific for O-methyl-L-tyrosine. Protein Sci. 2005 May; 14(5):1340-9.

    View in: PubMed

  46. Wang L, Schultz PG. Expanding the genetic code. Angew Chem Int Ed Engl. 2004 Dec 17; 44(1):34-66.

    View in: PubMed

  47. Wang L, Jackson WC, Steinbach PA, Tsien RY. Evolution of new nonantibody proteins via iterative somatic hypermutation. Proc Natl Acad Sci U S A. 2004 Nov 30; 101(48):16745-9.

    View in: PubMed

    Science

    News of the Week (Science, 2004, 306:1457)

    Nature Methods

    Research Highlights (Nat. Methods 2005, 2:87)

    F1000Prime Recommended

  48. Xie J, Wang L, Wu N, Brock A, Spraggon G, Schultz PG. The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination. Nat Biotechnol. 2004 Oct; 22(10):1297-301.

    View in: PubMed

    F1000Prime Recommended

  49. Wang L. Amersham Prize winner. Expanding the genetic code. Science. 2003 Oct 24; 302(5645):584-5.

    View in: PubMed

  50. Zhang Z, Smith BA, Wang L, Brock A, Cho C, Schultz PG. A new strategy for the site-specific modification of proteins in vivo. Biochemistry. 2003 Jun 10; 42(22):6735-46.

    View in: PubMed

  51. Liu H, Wang L, Brock A, Wong CH, Schultz PG. A method for the generation of glycoprotein mimetics. J Am Chem Soc. 2003 Feb 19; 125(7):1702-3.

    View in: PubMed

    Science

    Editors’ Choice (Science 2003, 299:1283)

    F1000Prime Recommended

  52. Mehl RA, Anderson JC, Santoro SW, Wang L, Martin AB, King DS, Horn DM, Schultz PG. Generation of a bacterium with a 21 amino acid genetic code. J Am Chem Soc. 2003 Jan 29; 125(4):935-9.

    View in: PubMed

  53. Wang L, Xie J, Deniz AA, Schultz PG. Unnatural amino acid mutagenesis of green fluorescent protein. J Org Chem. 2003 Jan 10; 68(1):174-6.

    View in: PubMed

    F1000Prime Recommended

  54. Wang L, Zhang Z, Brock A, Schultz PG. Addition of the keto functional group to the genetic code of Escherichia coli. Proc Natl Acad Sci U S A. 2003 Jan 7; 100(1):56-61.

    View in: PubMed

    Science

    Editors’ Choice (Science, 2003, 299:1283)

  55. Santoro SW, Wang L, Herberich B, King DS, Schultz PG. An efficient system for the evolution of aminoacyl-tRNA synthetase specificity. Nat Biotechnol. 2002 Oct; 20(10):1044-8.

    View in: PubMed

  56. Chin JW, Santoro SW, Martin AB, King DS, Wang L, Schultz PG. Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli. J Am Chem Soc. 2002 Aug 7; 124(31):9026-7.

    View in: PubMed

  57. Zhang Z, Wang L, Brock A, Schultz PG. The selective incorporation of alkenes into proteins in Escherichia coli. Angew Chem Int Ed Engl. 2002 Aug 2; 41(15):2840-2.

    View in: PubMed

  58. Chin JW, Martin AB, King DS, Wang L, Schultz PG. Addition of a photocrosslinking amino acid to the genetic code of Escherichiacoli. Proc Natl Acad Sci U S A. 2002 Aug 20; 99(17):11020-4.

    View in: PubMed

  59. Wang L, Brock A, Schultz PG. Adding L-3-(2-Naphthyl)alanine to the genetic code of E. coli. J Am Chem Soc. 2002 Mar 6; 124(9):1836-7.

    View in: PubMed

    F1000Prime Recommended

  60. Wang L, Schultz PG. Expanding the genetic code. Chem Commun (Camb). 2002 Jan 7; (1):1-11.

    View in: PubMed

  61. Wang L, Schultz PG. A general approach for the generation of orthogonal tRNAs. Chem Biol. 2001 Sep; 8(9):883-90.

    View in: PubMed

    F1000Prime Recommended

  62. Wang L, Brock A, Herberich B, Schultz PG. Expanding the genetic code of Escherichia coli. Science. 2001 Apr 20; 292(5516):498-500.

    View in: PubMed

    F1000Prime Recommended

  63. Wang L, Magliery TJ, Liu DR, Schultz PG. A New Functional Suppressor tRNA/Aminoacyl-tRNA Synthetase Pair for the in vivo Incorporation of Unnatural Amino Acids into Proteins. J Am Chem Soc. 2000; 122(20):5010-5011.
    View in: PDF

  64. Song JQ, Li CZ, He HX, Chen Y, Wang L, Liu ZF. Fabrication of Gold Nanostructures on Graphite Using Atomic Force Microscope. Molecular Crystals and Liquid Crystals. 1997; 294(1):51-54.

  65. He HX, Li CZ, Song JQ, Mu T, Wang L, Zhang HL, Liu ZF. Force Titration of a Carboxylic Acid Terminated Self-Assemble Monolayer Using Chemical Force Microscopy. Molecular Crystals and Liquid Crystals. 1997; 294(1):99-102.

  66. Chen Y, Wang L, He HX, Liu ZF. Developing High Resolution Electrical Probing System Based on Atomic Force Microscopy. Molecular Crystals and Liquid Crystals. 1997; 294(1):91-94.

  67. Zhu B, Zhang P, Wang L, Liu Z. NOTE J Colloid Interface Sci. 1997 Jan 15; 185(2):551-3.

    View in: PubMed